The ruling came as the three men who leaked a recording that they claim shows Anwar with a prostitute identified themselves as two ex-government officials and a businessman.
Anwar insists both the sodomy charge and the video were fabricated by the government to crush the rise of his opposition alliance, which made significant gains in 2008 elections.
The court decision Wednesday was a reversal of its own recent ruling that a toothbrush, towel and water bottle that Anwar kept during police detention could not be submitted as evidence. A chemist testified that DNA on those items matched that of semen discovered on the man who accused Anwar of sodomy.
The evidence is a vital part of the prosecution’s effort to prove Anwar had sex with his 25-year-old former aide.
Anwar faces up to 20 years in prison if convicted of sodomy, a crime in Muslim-majority Malaysia.
The High Court decided earlier this month the DNA evidence was not obtained legally during Anwar’s brief detention in 2008. It reversed its ruling after hearing new testimony from police.
Anwar refuses to voluntarily provide a DNA sample because he fears authorities will tamper with it.
Without DNA evidence, the prosecution’s case hinges on testimony by Anwar’s ex-aide, Saiful Bukhari Azlan, who claims Anwar coerced him into having sex in 2008.
Prosecutors plan to wrap up their case Thursday.
Anwar, a married 63-year-old with six children, has also been battling to convince the public that he is not the man seen having sex with a woman in a video shown to several Malaysian journalists Monday.
A former Malaysian state chief minister and a businessman held a news conference Wednesday to confirm they and an ex-senator were the people who leaked the video. The businessman said he found the recording in a hotel room last month, indicating he did not know who taped it.
“We showed the video to expose ... the person who could be the next prime minister of Malaysia,” said Rahim Thamby Chik, a former high-ranking official in Malaysia’s ruling party.
The video has not been publicly circulated. Johari Abdul, an opposition member of Parliament, has seen the footage after receiving an invitation from the men, but he insisted the man featured in it looked more plump than Anwar and had broader shoulders.
Debate over the video could hurt the opposition in an April 16 state election. Prime Minister Najib Razak’s ruling coalition hopes to easily retain control of the Sarawak state legislature on Borneo island to boost its confidence ahead of national elections widely expected within a year.